Alzheimer’s Disease (AD) Model: Gamma-secretase Activity Assay Cell Line

Description Gamma Secretase Activity Assay Cell Line is an Alzheimer’s Disease in vitro model to identify γ-secretase activity inhibitors. It consists in U2OS cell line stably expressing green fluorescent APP-C99 construct, which contains the γ-secretase cleaving site. To perform the assay, cells are incubated with test compounds during 24 hours to evaluate their effects on APP Processing. After incubation, nucleus could be stained with DAPI and retained fluorescent APP vesicles are detected by fluorescence using image analysis algorithms. Cells Online offers this assay as a stable cell line in cryopreserved vials. It is guaranteed that these cells will expand in more than 30 passages following the cell culture manual. 2 vials, 3 x 10^6 cells/ vial