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Course 1: Real-Time PCR (qPCR)

Course 1: Real-Time PCR (qPCR)

💡 Learn how to detect and quantify nucleic acids in real-time

What you’ll learn:

  • Principles of fluorescence detection (SYBR Green, TaqMan)

  • qPCR vs conventional PCR

  • Setting up standard curves and quantification

  • Ct/Cq values interpretation

  • Avoiding false positives/negatives

  • Efficiency, R², and melt curve analysis

  • Troubleshooting low amplification or non-specificity

Who is it for?

🔹 Clinical labs, diagnostic labs, research teams using RT-PCR for gene expression, pathogens, SNPs, etc.

🕒 Duration: 3 hours

📜 Certificate: Yes – completion & skills certificate

🧪 Includes: downloadable protocols + virtual qPCR simulation

50m 2 steps
qPCR Training Course : Principles, Techniques and Data Analysis

qPCR Training Course : Principles, Techniques and Data Analysis

Mastering qPCR: From Basics to Advanced Applications
Learn how to design, execute, and analyze quantitative PCR (qPCR) experiments with precision and confidence. This comprehensive qPCR training course covers everything from fundamental principles and reagent selection to advanced data analysis and troubleshooting strategies. Whether you are a beginner starting your first qPCR experiment or a professional aiming to optimize workflows, you’ll gain practical, hands-on knowledge to improve accuracy, reproducibility, and efficiency. Ideal for researchers, molecular biologists, diagnostics specialists, and laboratory technicians, this course provides step-by-step guidance, real-world examples, and expert tips to help you achieve reliable and publication-ready results.
Intermediate
1h 50m 1 steps

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