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ExoQuick-LP for lipoprotein pre-clear & exosome isolation

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ExoQuick-LP is a powerful and efficient solution for isolating exosomes while simultaneously removing lipoprotein contaminants from plasma or serum samples. This innovative reagent ensures high-purity extracellular vesicle (EV) recovery, making it ideal for downstream applications such as proteomics, RNA analysis, and biomarker discovery. With its simple, user-friendly protocol, ExoQuick-LP saves time and ensures consistent, reproducible results, all without the need for ultracentrifugation. Perfect for researchers working with small sample volumes, ExoQuick-LP optimizes your workflow for reliable exosome isolation every time.

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Conditions générales
Garantie satisfait ou remboursé de 30 jours
Expédition : 2-3 jours ouvrables

Tutorial: How to Use ExoQuick-LP for Lipoprotein Pre-Clear and Exosome Isolation: 

Step-by-Step Guide for Exosome Isolation with ExoQuick-LP

  1. Prepare Your Sample
    • Collect plasma or serum samples using proper collection methods.
    • Centrifuge the sample at 3000 x g for 10–15 minutes to remove any cellular debris or aggregates.
    • Transfer the clarified plasma or serum to a fresh tube.
  2. Add ExoQuick-LP Reagent
    • Add the appropriate volume of ExoQuick-LP reagent to the sample based on the kit instructions.
    • Mix the solution thoroughly by inverting the tube several times.
  3. Incubate the Mixture
    • Incubate the sample and ExoQuick-LP mixture at 4°C for 30 minutes. This allows the reagent to bind lipoproteins and initiate the precipitation of extracellular vesicles.
  4. Centrifuge to Isolate the EV Pellet
    • Spin the tube at 10,000 x g for 10 minutes to pellet the exosomes and remove lipoprotein contaminants.
    • Carefully discard the supernatant, ensuring the pellet remains intact.
  5. Resuspend the Pellet
    • Resuspend the exosome pellet in a suitable buffer (e.g., PBS or a specialized lysis buffer) for downstream applications.
    • Mix gently to ensure the pellet is fully dissolved.
  6. Proceed with Your Research
    • Use the purified exosomes for RNA extraction, protein analysis, or any other application requiring high-purity EVs.

Why Use ExoQuick-LP for Exosome Isolation?

  • Efficient Lipoprotein Removal: Ensures that your exosome preparations are free of lipoprotein contaminants.
  • Optimized for Plasma and Serum: Tailored specifically for use with these sample types.
  • User-Friendly Protocol: Simplifies exosome isolation without ultracentrifugation.
  • High-Yield and Purity: Delivers consistent, reproducible results with minimal hands-on time.